ihc for p53  (Novocastra)

Journal: ACS Omega

Article Title: Identification of a Natural Small Molecule Dauricine for Glioma Therapy through Targeting p53 and VEGFA Pathways

doi: 10.1021/acsomega.5c01334

Figure Lengend Snippet: Upregulation of p53 and VEGFA in human cancers. (A,B) Comparison of p53 ( n = 443) or VEGFA ( n = 452) expression in different types of cancer analyzed by Oncomine. (C,D) p53 and VEGFA expression in different primary tumors and corresponding normal tissues analyzed by Metabolic gEne RApid Visualizer. (E,F) p53 and VEGFA expression in normal brain tissue and corresponding glioblastoma tissue as revealed by IHC from Human Protein Atlas. (G,H) Survival probability analyses of p53 and VEGFA expression by Kaplan–Meier plotter ( n = 153).

Article Snippet: Furthermore, IHC analyses of p53 and VEGFA expression from Human Protein Atlas ( https://www.proteinatlas.org/ ) also indicated that both p53 and VEGFA were upregulated in gliomas ( E,F).

Techniques: Comparison, Expressing

Journal: ACS Omega

Article Title: Identification of a Natural Small Molecule Dauricine for Glioma Therapy through Targeting p53 and VEGFA Pathways

doi: 10.1021/acsomega.5c01334

Figure Lengend Snippet: Molecular docking of dauricine and the p53 core domain as well as VEGFA. (A) Dauricine binds to the L1/S3 pocket of p53, forming two hydrogen bonds with Gln144 and Pro142 from Sheet3. P53 is shown in blue, and dauricine in yellow. The hydrogen bonds are shown with gray dashed lines. The rest part of the dauricine is hidden for the clarity of view. (B) Dauricine interacts with Ser116, Cys124, and Thr140 in the L1/S3 pocket of p53. (C) Dauricine binds to the L1/L3 region of VEGFA, interacting with Phe36 and Ile46 in the Loop1 (L1) region. (D) Dauricine binds to the Loop3 (L3) region of VEGFA, forming a hydrogen bond with His86 and interacting with Lys84 as well as Pro85.

Article Snippet: Furthermore, IHC analyses of p53 and VEGFA expression from Human Protein Atlas ( https://www.proteinatlas.org/ ) also indicated that both p53 and VEGFA were upregulated in gliomas ( E,F).

Techniques:

Journal: ACS Omega

Article Title: Identification of a Natural Small Molecule Dauricine for Glioma Therapy through Targeting p53 and VEGFA Pathways

doi: 10.1021/acsomega.5c01334

Figure Lengend Snippet: Dauricine upregulates p53 expression, downregulates VEGFA expression, and induces apoptosis in glioma cells. (A) Western blot analyses of p53 and its downstream genes. (B) Gray scale statistics of protein expression in U87 cells. (C) Western blot analyses of VEGFA and its downstream genes. (D, E) Gray scale statistics of protein expression in C6 cells (* P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001 vs control, n = 3).

Article Snippet: Furthermore, IHC analyses of p53 and VEGFA expression from Human Protein Atlas ( https://www.proteinatlas.org/ ) also indicated that both p53 and VEGFA were upregulated in gliomas ( E,F).

Techniques: Expressing, Western Blot, Control

Journal: ACS Omega

Article Title: Identification of a Natural Small Molecule Dauricine for Glioma Therapy through Targeting p53 and VEGFA Pathways

doi: 10.1021/acsomega.5c01334

Figure Lengend Snippet: Pathological analyses of C6 glioma xenograft tissue. (A) HE staining was performed on three groups of C6 tumor tissue sections. Photos were taken under the microscope at 100× and 400×. Blue-violet staining indicates tumor cell nuclei. (B) IHC analyses of the tumor sections. p53 and VEGFA were detected in the subcutaneous xenograft tumor sections of nude mice. Brown staining indicates positive signals. (C,D) Statistical analyses of p53 and VEGFA expression in IHC staining of the xenograft (* P < 0.05, ** P < 0.01, **** P < 0.0001 vs Control, n = 6).

Article Snippet: Furthermore, IHC analyses of p53 and VEGFA expression from Human Protein Atlas ( https://www.proteinatlas.org/ ) also indicated that both p53 and VEGFA were upregulated in gliomas ( E,F).

Techniques: Staining, Microscopy, Expressing, Immunohistochemistry, Control